INCF plasmids responsible by dissemination of blaKPC gene among enterobacteriaceae
نویسندگان
چکیده
Methods A total of 22 isolates, K. pneumoniae (n=20), Escherichia coli (n=1) and Enterobacter aerogenes (n=1), were collected from a university hospital, in Lisbon, Portugal. MIC were determined by Etest and interpreted according to Clinical and Laboratory Standards Institute guidelines. PCR was performed with primers designed to amplify blaSHV, blaTEM and blaKPC genes. Molecular typing was performed by M13-PCR fingerprinting and in representative strains by Multilocus Sequence Typing (MLST). Replicon typing was used to define plasmid incompatibility groups (Inc).
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